IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/07/Min Lin

01:52
GEL to assess the digestion

CIP the 6 rbs vectors.
Add to the digestion product 5ul CIP Buffer and 1ul CIAP

Run a GEL to recycle the insert of tetR.
The Hole is too large, the band is missing. (Never use the largest Cone for 20ul recycle any more)

03:24
Digest the tetR plasmid again.