IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/06/24/Rencheng Gao

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16:21
Double-enzyme digestion: BtoB plasmid (090623) with EcoRI and PstI

16:30
Pick Colony 1 from the plate of BtoB (090623) and cultivate the bacteria in LB medium with Kan.

20:00
Gel electrophoresis: the product of double-enzyme digestion.

20:15
Double-enzyme digestion: BtoB plasmid (090623) with EcoRI and PstI