IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Core/2009/07/13/Zhangs
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Double digestion for T7ptag + 1-23L insert
Unable to differentiate T7ptag + 1-23L insert and the backbone
Then digest T7ptag+1-23L insert with 3 enzymes
System: 20uL
| 1.5μL | XbaI |
| 1.5μL | PstI |
| 2μL | 10×M buffer |
| 3μL | plasmid |
| 1.5uL | HindIII |
| 10.5μL | ddH2O |
| 20μL | Total |
2 samples in totall: T7ptag+T1, T7ptag+T2.
Elcetrophoresis to pure insert.
Sample loading order: T7ptag+T1, T7ptag+T2, vector plasmid control, Marker.
The insert has been cut out.
