IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/19/Zhangs

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Store the spawns of T7p+5J+CI+1-23L ①~⑤
Bacteria Liquid PCR to identify
System (Master mix is exhausted)

0.5μL Tag enzyme
1μL 10× buffer
2μL dNTP
0.25μL Forward primer
0.25μL Reverse primer
6μL ddH2O
Template (Bacteria Liquid)
10μL Total

Electrophorsis
Result (from left to right)
Marker, T7p+5J+CI+1-23L ①~⑤, Control
PKU 20090719 Zhangs 1.JPG
The T7p+5J+CI+1-23L ②,④,⑤ are target plasmids.