IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/07/26
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Date: 7/26/14 People in lab: Emily PuleoTitle: Miniprep and nanodrop Start Time: 9:30 am Purpose: Purification of plasmids that most probably have hmp and testing of quality of such Protocol: LTM Ed. 2 Miniprep and nanodrop Exceptions: Products:
Results: Notes: Forgot to mix MP1 and MP2 so retested Stop Time: 11:30 am Next: digest with EcoRI to confirm presence of hmp Date: 7/26/14 People in lab: Emily PuleoTitle: Digestion Start Time: 11:40 am Purpose: To check for the presence of hmp Protocol: LTM Ed. 2 Digestion Exceptions: Products:
Results: Notes: Stop Time: 1:35 pm Next: Run a gel to determine size Date: 7/26/14 People in lab: Emily PuleoTitle: Gel Electrophoresis Start Time: 1:45 pm Purpose: To check product size from digestion Protocol: LTM Ed. 2 Gels Exceptions: Products:
Products: Results: Notes: Stop Time: 2:30 pm Next:
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