IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/04/14

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Date: 04/14/14 People in lab: Kelsey Crossen

Title: PCR norCB

Start Time: 2:30PM

Purpose: To amplify norCB gene from P. aeruginosa

Protocol: LTM ed. 2 Exceptions: 1. Annealing temp: 55C


Sample Label Description Source Label Quantity
norCB 4/14 Amplified norCB with prefix and suffix P aeru 2 3/3 1



Stop Time: 5:00 PM

Next: Run gel to check for PCR product

Date: 04/14/14 People in lab: Kelsey Crossen

Title: Gel electrophoresis of norCB PCR

Start Time: 6:50 PM

Purpose: To check for successful PCR

Protocol: LTM ed. 2

Well 1 2 3 4 5 6 7 8
Sample Ladder norCB 4/14

Results: No PCR product visible

Stop Time: 8:00 PM

Next: Try low temp PCR (annealing Temp 40C)