IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/04/14

Cyanobacteria Mediated Filtration of Coal Flue Gas

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Date: 04/14/14 People in lab: Kelsey Crossen Title: PCR norCB Start Time: 2:30PM Purpose: To amplify norCB gene from P. aeruginosa Protocol: LTM ed. 2 Exceptions: 1. Annealing temp: 55C Products: Sample Label Description Source Label Quantity norCB 4/14 Amplified norCB with prefix and suffix P aeru 2 3/3 1 Results: Notes: Stop Time: 5:00 PM Next: Run gel to check for PCR product Date: 04/14/14 People in lab: Kelsey Crossen Title: Gel electrophoresis of norCB PCR Start Time: 6:50 PM Purpose: To check for successful PCR Protocol: LTM ed. 2 Well 1 2 3 4 5 6 7 8 Sample Ladder norCB 4/14 Results: No PCR product visible Stop Time: 8:00 PM Next: Try low temp PCR (annealing Temp 40C)