# IGEM:MIT/2009/PCB Spirulina

The resulting spectrum received from the spec is shown:

Here is an example of what is a good read out from the Quail lab.

The concentration is found by this formula:

${\displaystyle C(mM)={\frac {A_{680}}{37.9}}*{\begin{cases}100(for1:100)\\1000(for1:1000)\end{cases}}}$

We didn't trust the pathlength correction given to us by this spec so we ran the 1:100 and 1:1000 dilutions in the Stephanopolous lab, and here are the results that we got:

1:100 - A680 = 1.208
1:1000 - A680 = .425

After completing the calculation with the 1:1000 dilution, we got a concentration of 11.21372 mM, and we have a 4.5 mL solution.