IGEM:MIT/2007/Protocols

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General Lab Protocols

  • [[../Notebook/2007-6-11#General_Lab_Do_s_and_Don’t_s|General Lab Do's and Don'ts]]
  • [[../Notebook/2007-6-12#Nanodrop (~20ng/ul) Protocol|Calculating Optical Density; Nanodrop Protocol]]
  • [[../Notebook/2007-6-12#Obtaining a Cast|Making a Cast]]
  • [[../Notebook/2007-6-11#made_1_liter_1%_agarose_solution|Making Agarose Solution]]
  • [[../Notebook/2007-6-12#Protocol for Preparing Agarose Gel|Making an Agarose Gel]]
  • [[../Notebook/2007-6-12#Protocol for Preparing to Run a Gel Electrophoresis|Preparing a Gel Electrophoresis]]
  • [[../Notebook/2007-6-12#Protocol for Running a Gel Electrophoresis|Running a Gel Electrophoresis]]
  • [[../Notebook/2007-6-13#Making_LB_Amp_Agar_Plates|Making LB Amp Agar Plates]]
  • [[../Notebook/2007-6-11#innoculate_5ml_LB/Amp_with_bact._colonies_(EGFP_and_NNX)|Innoculating LB with bacteria]]
  • [[../Notebook/2007-6-12#Protocol for Imaging Result and Gel Diagnostic|Gel Imaging]]

DNA Work

  • [[../Notebook/2007-6-12#Mini Prep|Mini Prep]]
  • [[../Notebook/2007-6-18#Mini-prep|Mini Prep v.2.0]]
  • [[../Notebook/2007-6-12#PCR_amplification_of_EGFP|PCR Amplification]]
  • [[../Notebook/2007-6-12#PCR_purify(microcentrifuge)|PCR Purification in a Microcentrifuge]]
  • [[../Notebook/2007-6-12#PCR Cleanup|PCR Cleanup]]
  • [[../Notebook/2007-6-12#Notes for Double Digestion|Double Digestion]]
  • [[../Notebook/2007-6-13#Ligation|Ligation]]
  • [[../Notebook/2007-6-13#Purifying_the_DNA|Purifying the DNA]]
  • [[../Notebook/2007-6-14#Transformation_Protocol|Transformation]]
  • [[../Notebook/2007-7-19#Colony PCR Protocol (OWW, Knight)|Colony PCR (OWW, Knight)]]
  • Phosphatase treatment of linearized vector