IGEM:MIT/2007/Notebook/2007-7-27

Agenda

• dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
  • Miniprep LC's of Mer/GFP
• Finish Western Blot
• Order EC20 oligos

EC_20 Oligos

• Ordered with IE-HCLG purification, should arrive Tuesday

Polystyrene Assay

Wells:
(Done in Triplicate)
CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced)

Diagram of the Polystyrene Assay Protocol

• 
  E-5a
• 
  E-5b
• 
  E-5c
• 
  E-6a
• 
  E-6b
• 
  E-6c
• 
  E-7a
• 
  E-7b
• 
  E-7c
• 
  E-7d
• 
  E-7e
• 
  E-7f
• 
  E-8a
• 
  E-8b
• 
  E-8c
• 
  E-8d
• 
  E-8e
• 
  E-8f
• 
  Control 1
• 
  Control 2
• 
  Control 3
• 
  Image 21

Diagram of the Polystyrene Assay Protocol

Western Blot Results

A pdf of the blots