IGEM:MIT/2007/Notebook/2007-6-15

Grad Advisors

• Morning: Debbie, Forrest
• Afternoon: Brian

LAB WORK

Previous night:

• Analyze plates
• innoculate 4ml LB/Amp

Day of:

• Mini-prep plasmid DNA

Results from last night's incubation

Contents    Volume    Label    # of Colonies    
U V         (2 µL)    I        67
ddV         (5 µL)    A        0
ddV + ddI   (5 µL)    B        0
dV          (5 µL)    C        28               
dV + dI     (5 µL)    D        49
ddV         (10 µL)   E        0
ddV + ddI   (10 µL)   F        5
dV          (10 µL)   G        0
dV + dI     (10 µL)   H        5

• All plates with colonies have feeder colonies

Plan

1. Plate and look at them.
2. At least a couple colonies.
3. if lots, pick 2 colonies from each; doesn't matter 5 ul or 10 ul. Just need 4 different types
4. One colony into one tube, and repeat. So we have 11 innoculation tubes; 2 for each type and all 5 for ddV/ddI.
5. Put LB amp into glass tubes with metal caps (flamed)
6. Loop and insert colonies.
7. Place plates in fridge
8. Place tubes in 37°C room overnight
9. Store in refigerator for mini-prep on monday

Tubes:

• 2: U V
• 2: dV
• 2: dV + dI
• 5: ddV + ddI