IGEM:MIT/2006/Notebook/2008-2-8

Alcohol dehydrogenases

• adhP - similar to ADH1 from yeast[1]
• adhE - ineffective on branched alcohols
• YqhD - some activity on isoamyl alcohol but not 3-methylbutanal

References

1. Shafqat J, Höög JO, Hjelmqvist L, Oppermann UC, Ibáñez C, and Jörnvall H. An ethanol-inducible MDR ethanol dehydrogenase/acetaldehyde reductase in Escherichia coli: structural and enzymatic relationships to the eukaryotic protein forms. Eur J Biochem. 1999 Jul;263(2):305-11. DOI:10.1046/j.1432-1327.1999.00323.x |
2. Sulzenbacher G, Alvarez K, Van Den Heuvel RH, Versluis C, Spinelli S, Campanacci V, Valencia C, Cambillau C, Eklund H, and Tegoni M. Crystal structure of E.coli alcohol dehydrogenase YqhD: evidence of a covalently modified NADP coenzyme. J Mol Biol. 2004 Sep 10;342(2):489-502. DOI:10.1016/j.jmb.2004.07.034 |