IGEM:MIT/2006/Notebook/2007-7-19
To Do Today
1. Add PCNB to all GC samples- DONE
2. Run all EIGHTEEN GC samples- DONE
3. Take out plate of IlvE- strain- DONE
4. Take out new LCs of J45120 and J45181- DONE
5. Adjust the pH of Tom's media as Tom suggested- DONE (looks to be ~7.7)
6. Grow up the same cultures on Tom's pH-adjusted media- DONE
7. Add 50 uL water to tube containing Japanese plasmid- DONE
8. Nanodrop the contents of the tube containing Japanese plasmid- DONE (21.6 ng/uL DNA)
Results
J45120
Hour-----------OD600------------Methyl Salicylate (ppm)-------Methyl Salicylate (ppm)/OD600
5--------------0.14-------------0.0---------------------------0.00
6--------------0.42-------------0.0---------------------------0.00
7--------------0.84-------------0.0---------------------------0.00
8--------------1.38-------------1.0---------------------------0.72
9--------------1.84-------------23.1--------------------------12.6
10------------2.20--------------28.7--------------------------13.1
11------------2.26--------------26.1--------------------------11.6
12------------2.40--------------27.5--------------------------11.5
13------------2.48--------------18.8--------------------------7.58
J45181
Hour----------OD600-------------Methyl Salicylate (ppm)-------Methyl Salicylate (ppm)/OD600
5--------------0.24-------------0.0---------------------------0.00
6--------------0.50-------------1.1---------------------------2.20
7--------------0.92-------------8.7---------------------------9.46
8--------------1.46-------------18.5--------------------------12.7
9--------------1.82-------------22.3--------------------------12.3
10------------2.30--------------25.9--------------------------11.3
11------------2.20--------------24.8--------------------------11.3
12------------2.38--------------24.8--------------------------10.4
13------------2.52--------------18.3--------------------------7.26