IGEM:MIT/2006/Notebook/2007-6-22

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Things to Do

1. GC sample extraction from cultures last night (add methyl salicylate during this)- DONE

2. Add PCNB to samples

3. Write back Prather- DONE

4. Research chorismate pathway/possible overly expressive strains- DONE (for now)

Links:

Chorismate pathway: http://biocyc.org/ECOLI/NEW-IMAGE?object=ALL-CHORISMATE-PWY

Overproduction of tryptophan: http://aem.asm.org/cgi/reprint/38/2/181

Screening procedure to overproduce chorismate: http://www.freepatentsonline.com/6030819.html

Overproduction of aromatic amino acids: http://www.patentstorm.us/patents/5776736-claims.html

Phenylalanine: a bad option: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3C-4DV1KTR-2&_user=501045&_coverDate=02%2F09%2F2005&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000022659&_version=1&_urlVersion=0&_userid=501045&md5=a78dec0c6826cd67fe11be414d51c3d5#SECX7

5. Prep for plate reader experiment via procedure outlined yesterday

6. Resend Tom the email inquiring about the GC- DONE

7. Run GC samples

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