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1. Dilute the osmY and control structures to 1:250 in 5 mL culture and grow up for 1 hr- DONE

2. Run the osmY and control structures on the plate reader- DONE

3. Make LCs of the three 170.320 colonies- DONE

4. Redigest 170- DONE

5. Religate and transform 170.320 mix- DONE

  • Stephen - I put cells in warm room at 4:25...so plate after 5:25 I guess? I left plates out on the bench by the hot water bath. thanks! -Veena

6. Run 170 cut on gel