IGEM:MIT/2006/Notebook/2007-1-26
NO COLONIES FROM J45800 ligations- NO!!!
Plan
1. Submit samples of J45600 hopefuls- DONE
2. Order ether and pentane for extraction of isoamyl acetate- DONE
3. Analyze/organize plate reader data- DONE (results were okay, will consult with Barry but my inclination is that we may need to repeat the experiment to allow for greater growth among the cultures)
4. Redigest- DONE, ligate- DONE, and transform J45320/J45170 mixes- DONE
5. Smell J45600 hopefuls- DONE
6. Talk to Lily about the GC results- DONE
7. Contact Li Li about using the original GC for the methyl salicylate samples- DONE
8. Run the J45320 an J45170 cut products on a gel- DONE (170 doesn't look cut, maybe we'll get lucky, if not, will redigest tomorrow)
9. Regrow osmY and control structures for the plate reader tomorrow- DONE