IGEM:MIT/2006/Notebook/2006-8-28
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completed
- re-ordered pms primers to PCR out the entire SA-generating device from plasmid pE3/pE3R
- miniprepped 12 30.Bat2.30.THI3 mutagenesis attempts -- ran gel: 2 correct
- threw out bad ones
- analyzed sequencing:
- one shuttle vector construct correct pUCP22-100-C
- Q-E0840-A, Q-119-C, Q-199-C correct
- threw out bad ones
- LCed new CHA0 pseudomonas strain, YYC912, pE3, pE3R
- try to figure out/organize mystery tubes/glycerols using digest/ligation sheets and plates
- found lots of poorly labeled tubes (i.e. no date or description)