IGEM:MIT/2006/Notebook/2006-8-23
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Things done today off the top of my head:
1. Miniprepped the 30.bat2.30.thi3 mutants, the pchba.term mutants, and the osmY.inverter.xxx structures.
2. Digested both the 30.bat2.30.thi3 mutants and pchba.term mutants and ran on a gel.
3. Ligated and transformed the correctly cut pchba.terms with rbss.
4. Analyzed and graphed the osmY.E0840 results. It was a huge success.
5. Glyceroled the pseudomonas strain, the correct smelling indole-knockouts, and the top10 osmY.inverter.xxx structures.
6. Transformed the osmY.inverter.xxx structures into recently-made competent indole-knockout cells.