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to do

  1. miniprep all LCs and sequence -done
  2. check incoming sequence results (and judge semi-solid parts in freezer)
  3. site-directed mutagenesis for pchBA-B0015 and correct TH13 -done
  4. digest osmY (long new, short new, short old) with ES and 3-part assemble into biobrick format (plus with E0840) -done
  5. get new registry uncut backbones (A/K and A/T, etc.) and cut with EP -done
  6. acknowledgement link, and iGEM main page summary/picture
  7. ligations/transformations -done
  8. ppm
  9. glycerol updating