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  1. Digest 250/400 double plasmids with XP -done, KB, VV
  2. Repeat digest of missing 250 with ES -done, KB
  3. Ligate final constructs -done, KB
  4. Transform final constructs in Jason's competent cells -done, KB
  5. Start an LC of plain IK cells in plain LB -done, KB


  1. Locate iGEM glycerol boxes and put into Grossman Lab -done, AG
  2. Make an estimate of plates needed for streaking parts -done, AG