IGEM:MIT/2006/Autotrophic E.coli/Find metabolite

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  • Possible routes to functionally test Calvin Cycle in isolation (without proteorhodopsin system)
    • D-erythrose-4-phosphate (available from Sigma, but very expensive and impure...) -- side note, interestingly, DE4P is apparently a vitamin B6 precursor in K-12, which may have something to do with why Ichiro said over the phone that they had to supplement their RuBisCO/prkA cultures with a small amount of LB or vitamin B6.