IGEM:MIT/2005/Signal processing

POC

Ray

Function

• To eliminate noise from the incoming signal (delivered by the sensor group)
• To deliver a relatively clean and (usually) modular input to the actuator group

Ideas

• Continuous to discrete (C/D) converter
• Thresholding based on complementary mRNA competition

Current Status

• Threshold device:
  • All parts obtained, and are currently undergoing assembly (ready by monday probably)
    • I1030 with B0015 --> will be complete
    • I1031 with B0015 --> needs R0010/11
    • I1032 with B0015 --> needs R0010/11
    • E0840 with R0051 --> will be complete

• C/D: Still looking for appropriate proteins (shelved)

Device Depiction

• Thresholding device:
  • Sample graphs here: [1]

• C/D converter:

Device Parts

• Threshold: Have been specified and ordered (see picture above)

Experiments

• Objectives:
  • Ensure TetR::cI fusion works
  • Ensure lambda::GFP fusion works
  • Ensure antisense inihibits cI
• Strategy:
  • Fix level of IPTG and then vary TetR levels to see the amount of cI needed to express a certain amount of GFP
  • Change IPTG level, then repeat previous step.

Issues/Questions

• Do cell lines naturally have TetR repressors? or do I need to insert them?
• Assembly of various devices: One plasmid? different plasmids?

Need Help With

Nothing.. Nada..

Wishlist

• Feedback mechanism

Notes

• This group has secondary priority to the rest. It will gain prominence after the other devices are ready.
• cI system has internal thresholding built into the system. We may not be able to tweak it for our own nefarious purposes.