IGEM:MIT/2005/Notes
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June 20, 2005
Divide & Conquer
- Sensor - External signal -> Internal Signal
- Ray
- Jessica
- Signal-Processor - Internal Signal Processing
- Annie
- Jenny
- Actuator - Internal Signal -> Output
- Maxine
- Will
- System Integration - What is this whole system doing?
- Jen
- Read EVERYTHING by KT
- Summarize relevant papers (Journal Club)
- Don't make us look stupid.
- Please stock BUG Lounge with food. We are hungry college students
- We need to edit the Registry easier to read. More details.
- Plan assembly of test constructs
- J07000: R0053, Q04121, Q04510, E0430
- J07001: R0053, Q04121, Q04400, E0430
- J07002: R0040, Q04121, Q04510, E0430
- J07003: Z0251, Q04121, Q04510, E0430
- J07004: Z0251, Q04121, Q04400, E0420
- Place order with Registry for parts to be synthesized
- As said in above bullet.
- We need R0010. Has CAP binding site.
- Prepare FACS samples for Thursday
- We will get cells from Drew's Lab.
- Order new parts from Blue Heron
- Specify on Registry!
- insulin - Karen Talmadge
- export tag
- etc?
- Specify on Registry!
- Order supplies
- Make stocks
Test Constructs
How are we going to test our system in its intermediate, and also its final stages?
- Flow cytometry
- Beta gal assay
- Microscopy (detect gfp, bgal, etc)
- Western blot (measure protein levels)
- insulin assay?
- use cells as indicator - as cell-cell signalling system!
- insulin receptor (research this)
- sender/receiver
- feedback (incorporate insulin output into regulating glucose - ultimately CAP - input)
- RT-PCR
- used to measure RNA levels
- Northern Blot
- used to measure RNA levels
June 15, 2005
- Familarize
- Glucose receptor (what is the signal cascade?)
- plasmid?
- chromosome?
- Insulin plasmid (read the paper about it)
- How is insulin produce from E. coli?
- Glucose receptor (what is the signal cascade?)
- Tags
- Sequence of insulin
- Apoptosis in E. coli?
- List of things to get for lab
- Insulin is in 2 chains, so we need to look at how we can regulate it. In vitro?