IGEM:MIT/2005/Input reception Experiments

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Input Reception

Questions

  1. Is our fluorescein dimer entering into the cell?
  2. Distance in space between flurs. -- might depend on 3D conformation/wobblyness
  3. Separate out single from double stranded

Experiments

  1. Lets throw the oligos in, wash the media, look for flur. and we can hope that means its diffused
    • control: normal fluor. -- don't touch the oligos just yet
    • READOUT: can we see it under a microscope? get antibody -- into cytoplasm? selectivly trash outer membrane? will's oligo thingy?