IGEM:MIT/2005/Future Experiments
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Pathway ToxR
- Test Expression of ToxR
- Biobrick ToxR gene
- Tag ToxR w/ antibody recognition/GFP
- Test for presence of ToxR w/ FACs
- Test Function of Pathway of ToxR
- Biobrick ctx promoter
- Suffix insertion of GFP into ctx promoter plasmid
- Test pH/temperature changes for presence of GFP
- Alternative? E. coli not sensitive to temp/pH changes?
- Test Fusion of ToxR
- [Marker][ToxR][Transmembrane Protein][Marker]
- Test for first marker
- Test for second marker
- If both present, entire gene is produced!
Pathway FecA
- Isolate FecA transduction gene sequence
- Find coding sequences for FecR, FecI, Rpo, TonB, Exb D, Exb B, GFP, and fec promoter
- Biobrick
- Screen a linker that work to link FecA and antibody
- Fuse antibody with FecA w/ diff. linkers to antibody
- Screen for signal
