IGEM:MIT/2005/Friday 8/1 Meeting Agenda

Input

• We don't know if the fluor. oligos/molecules are even entering the cell
• Check on mic. settings
• Check for autofluor?
• Controling the pH: fluor. is an indicator
• Maybe electroporation isn't the best idea
• Lets clean up our technique so we know how to test our system
• Lets talk to the advisors -- need some new plans

Head Receiver

• For the ToxR system, do we need TT?

ToxR

• atcc -- order primers
• start assemblies if parts arrive

FecA

• Lab:
  1. Making FecA', quickchange
  2. coordinating for making parts
• Ordering primers for fusion, and fecA promoter
• Gathering materials for transformation
  1. Xgal and IPTG for QuikChange
• Knockouts: getting constructs -- ASAP

Signal Processing

• An experiment will happen if assemblies arrive