IGEM:MIT/2005/Friday 7/22 Meeting Agenda and Minutes

SubTeam Updates

(see individual wiki pages for details)

1. Input Update (Maxine)
2. Head Receiver Unit Update (Jenny)
3. Receiver 1 Update (Will)
4. Receiver 2 Update (Annie)
5. Signal Processor Update (Ray)
6. Actuator Update (Jessica - out)

Discussion of Main Issues

(make list as we go along. add an issue if you want).

1. effect on viability: osmolarity of solution (pressure exerted on cell membrane) -- use sorbitol?
2. testing scFv: need a const. promoter with RBS -- biobrick -- do western!
   • jenny is point person, get materials to do western early next week
3. scFv linker-fusion issue
   • why is the linker different than other scFvs?
   • can we introduce another linker, to allow fusion to preserve geometry
   • can we still introduce linker using primers? expensive
4. Making knock outs: lambda red system. use a non kan, non amp resistance
   • Counter selectable markers:
     1. PurF (involved in uracil synthesis) -- grow in media containing 5'FOA, will be processed by PurF protein, keep cells from growing?
     2. PheS