IGEM:MIT/2005/Experimental Timeline
From OpenWetWare
Jump to navigationJump to search
- [[../July wk 3:/]]
1. Receive primers [math]\displaystyle{ \rightarrow }[/math] Make biobricks, with these [[../Techniques/]]
J07005 (phoA), J07006 (malE), J07012-14 (scFv 1-3), J07018 (FecA), J07019 (FecA promoter), FecR, FecI.
1.5 Make competent MC4100 cells, pour LB Kan plates, make freezer stocks of strains acquired from Registry
2. ToxR:
Can begin assembly of R0040::B0030, for use in parts J07024-28 (see August wk. 1)
3. Input Experiments : Verify that flourescin oligo's enter cells.
4. Assemble fecA promoter with GFP --> test (after J07019 complete)
- July wk 4:
FecA: Fuse scFv with FecA? ToxR:
- Aug wk 1:
ToxR: -Receive synthesized J07007 (ctx promoter), J07009 (ToxR') -Begin Assembly of: -J07011 (ctx promoter :: gfp reporter) -J07024-26 (promoter::RBS::ToxR'::scFv 1-3) -J07027-28 (Positive and Negative controls, promoter::RBS::ToxR'::PhoA/MalE)
- Aug wk 2:
- Aug wk 3:
- Aug wk 4:
- Sept wk 1