scFv.TT (J07012-14.B0015)
digest B0015 E/X, recipient
obtain digested scFvs E/S, prefix donor
gel purify B0015
ligate J07012-14.B0015
transform
overnights for miniprep
identify correct clones (digest), streak plates
overnights for freezer stock
freezer stocks scFv.B0015
scFvs: 4M5.3, S101A, 4420
biobrick ends
digest scFv + pSB1AK3-1 plasmid w/ E/S
gel purify each
ligate together
transform
overnights for miniprep
identify correct clones (digest)
overnights for freezer stock
freezer stocks
