IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/10/14

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  • Sent off the parts - K272001 and K272002 (in both 1A2 and 1C3 plasmid backbones) to the regisrty.
  • Inoculated colonies from the transformation plates of K272001 and K272002 (in both 1A2 and 1C3) in LB broth without antibiotics to check for the fluorescence with a control as DH5a cells
  • singly transformed K272001 and K272002 showed red and cyan respectively
  • Inoculated 2 colonies from each of the cotransformation plates in LB broth containing no antibiotics to image tomorrow