IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/09/27

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  • Restriction of S03879(1,2 PCR)(S); I13507(1,2 PCR)(X); I13502(1,2 PCR)(X); I13504(1,2 PCR)(X); K145151(9,10 PCR)(S)
  • Ligated S+I2; S+I7; K+I4
  • PCR of S+I2; S+I7; K+I4 with primers 1 and 2.
  • gel run: S+I2; S+I7; K+I4 (2 wells each).