IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/09/15

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  • Transformed J23119(C)(S/P)+S+I7 and J23119(C)(S/P)+S+I2
  • Gradient PCR set up for K145151(x2) (1 with primers 3 and 4; 1 with primers 9 and 10)
  • Gel run for this PCR
  • Gel order:
  • Row1: K145151(3,4)(1 to 12); blank; ladder
  • Row2: K145151(9,10)(1 to 12); blank; ladder
  • S03879 Gradient PCR (12 values)
  • PCR of I13502; E0420; I13507; E0430; I13504; S03879 with 1 and 2 primers
  • PCR of K145151; K156011; K143065 with B0034 Primers
  • Gel run order
  • Row1: Ladder; S03879(G1 through 12); S03879(1,2)
  • Row2: Ladder; I13502; E0420; I13507; E0430; I13504; Blank; K145151; K156011; K143065; Blank; J13002(1)(S/P); J13002(2)(S/P)