IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/09/13

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  • Gel run was performed
  • Gel order: E0420(E/X)(3); J23119(S/P)(1); J13002(S/P)(1); E0430(X/P); S03879(S/P)(1); J23119(S/P)(2); S03879(S/P)(2); J13002(S/P)(1); E0420(E/X)(1); E0420(E/X)(2); Ladder; Ladder; J13507(E/X)(PCR2); I13502(E/X/P)(PCR2); I13507(E/X)(1); I13507(E/X)(1)
  • Alkaline phosphatase I13507(3,4)(1)(E/X); S03879(3,4)(2)(S/P); I13502(3,4)(2)(E/X/P).
  • PCR purification of all the digested parts. Gel run of these.
  • Ligations of I13507(1)(E/X) and S03879(2)(S/P) ;; J23119(S/P)(2) and I13502(3,4)(2)(E/X/P) ;; S03879(2)(S/P) and I13502(2)(E/X/P)