IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/21

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  • PCR followed by Dpn1 digestion of S03879 (Primers- VF2 and VR)
  • The following ligations were performed and they were grouped as D set of Ligations
  • R0040 + E0420 -----> this is a part from the kit viz.I13600
  • R0040 + E0422 -----> this is a part from the kit viz.I13602
  • R0040 + B0034 -----> this is a part from the kit viz.J13002
  • J23119 + S03879 ---> J+S1 (Ligated using Standard assembly)
  • J23119 + S03879 ---> J+S2 (Ligated using Standard assembly)
  • Inoculated colonies from transformants of C group of ligations and the remaining Ligations of group B viz. K272502 and K272500.