IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/04

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  • Lab space preparation.
  • Autoclaved glasswares, tips etc.
  • Obtained protocols for competent cell preparation
  • Prepared media and broth.
  • Prepared different stock solutions (Abs, CaCl, TBE)
  • Working concs of:
  • Amp= 100microgm/ml
  • Kan= 50microgm/ml
  • Chl= 25microgm/ml
  • Stock Solution of 1 M Calcium Chloride prepared.