IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/09/29
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Program used: IGEM1 (edited: elongation time = 45sec) /mistake: the program was run for 5 cycles with elongation time = 1min, then program running was stopped and running was started again with elongation time= 45sec)
S03879 (cut at S)+ PSB1A3 (cut at S); I13507 (cut at X) + PSB1A3 (cut at X); S03879 (cut at S) + I13507 (cut at X)
ladder-(empty)-PSB1A3-PSB1A3 (cut at X)-S03879 (cut at S)-I13507 (cut at X)- S03879 (cut at S)+ PSB1A3 (cut at S) - S03879 (cut at S) + I13507 (cut at X)- I13507 (cut at X) + PSB1A3 (cut at X) ///Doubt : 1A3 or 1A2????/// - Ladder
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