IGEM:Imperial/Results/I13208

11/8/06

Aims

The aim of this experiment was to test the Zinc Hypothesis. That is whether Zinc ions are required for AiiA Activity.

Extra Information

• The cultures were innoculated with a final AHL concentration of 5nM
• The I13208 were grown in the prescence of AHL for 2 hours
• The T9002 assays were assessed after a period of 2 hours
• Although a T9002 control for M9 was ommitted, previous experiments suggest a GFP/OD reading of 9500 for 0 AHL and 22000 for 5nM AHL after 3.5 hours
• Previous experiments also suggest a GFP/OD reading of 87000 for 5nM AHL when in LB culture after 3.5 hours.

Results

• Data File:
  • I132081 Test Data

Comments

• The experimental data gave unexpected results
  • The Flourescence/OD490 for both LB cultures was below that of the T9002 control
  • Only the -/Zn of the M9 cultures showed a significantly lower level of GFP/OD than the rest, this is unexpected

Conclusions

• No significant conclusion can be drawn from this experiment
• It should be repeated
• Controls using I13208 cells not in the prescence of AHL should be used
• Controls of T9002 in M9 should also be used