IGEM:Imperial/2010/Fast Response module

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Ideas
Signalling
2 components systems
logic gate
Vibrio cholera

Idea 1

The first idea, that seems to be feasible considering all the components, is the one below:

This is a bacterial two component system. On recieving of a signal, the Env Z receptor autophosphorylates. The phosphate is then passed on the respond element, a transcription factor called OmpR. OmpR is a molecule found naturally downstream of the signalling cascade from the EnvZ receptor. On phosphorylation the OmpR-P dimerise. Since each OmpR is fused with a split protease, when the OmpRs come together, the split protease (TEV) domains come together. This produces the active protease.


References:

  • all about EnvZ [1]
  • all about OmpR [2]
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