IGEM:Imperial/2010/Experiments1
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Experiments for the Output Amplification Model
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Output Amplification Model
- Determine the maximum concentration of sD that
cells can produce (in vivo): Compare activity with 2. - Determine how much XylE we need to produce
prior to adding Catechol (in vitro first, maybe in vivo
afterwards). In vivo IPTG: Compare cultures that
have less of it. - Determine XylE and TEV production (in vivo).
Use robot to induce production and measure
activities TEV (FRET pairs with TEV link). - Degradation of XylE (in vivo or in vitro if cell division
is not taken into account). Monitoring activity, then
approximate the concentration. Remove the IPTG. - Determine TEV kinetics (kcat, Km) on
XylE-GFP (in vitro).
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