Maddie was making two final testing constructs. One was XylE in the 3K3 testing vector and the other was... She also made a construct with XylE terminator in pSB1C3 ready to accept any promoter because we were waiting on Com and pVeg at the time.
Florian digested the surface protein linker genes to confirm their presence in pSB1C3. He then midiprepped the successful transformants.
Piotr PCR'd out XylE in the reverse orientation to insert into the LacI construct.