IGEM:IMPERIAL/2009/phases of project

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Phase 1: Protein production

Time regulation

1. NYMU 2008 Project

2. Genetic toggle switch
File:Timer-mutually repressive

3. Timer from Dr. Karen's talk
File:Timer-dr karen

Phase 2: Encapsulation

Cell Encapsulation

why encapsulate?

  • enhances cell resistance to freezing and freeze-drying (for storage)
  • added convenience and reduced packaging costs
  • longer stability and viability during storage

Various Cell encapsulation methods

natural encapsulation

encapsulation occurs naturally when bacteria trapped in own exo-secretions many lactobacilli secrete exo-polysaccharides, but most are insufficient to cover fully

Recent review on probiotic encapsulation methods

Phase 3: Plasmid deletion

Phase 4: Storage

Phase 5: Release