IGEM:IMPERIAL/2009/phases of project
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Phase 1: Protein production
Time regulation
2. Genetic toggle switch
File:Timer-mutually repressive
3. Timer from Dr. Karen's talk
File:Timer-dr karen
Phase 2: Encapsulation
Cell Encapsulation
why encapsulate?
- enhances cell resistance to freezing and freeze-drying (for storage)
- added convenience and reduced packaging costs
- longer stability and viability during storage
Various Cell encapsulation methods
natural encapsulation
encapsulation occurs naturally when bacteria trapped in own exo-secretions many lactobacilli secrete exo-polysaccharides, but most are insufficient to cover fully
Recent review on probiotic encapsulation methods
Phase 3: Plasmid deletion
- Bactoblood Project from Berkly 2007 - Plasmid Deletion and Freeze Drying Bacteria
- Programmed Cell Death - Use of anti-toxins and toxins to control cell death
- Cell Death mechanism by KU Leaven 2008 iGEM
- Articles about using restriction enzymes to lyse the host chromosome
- Class of Bacterial toxin
