IGEM:IMPERIAL/2009/alginate encapsulation

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Pure alginate encapsulation

resistance to acidity

entrapment of bifidobacteria in alginate microspheres
diameters of 40–80 μm

  • insignificant protection

larger alginate (1–3 mm) microspheres

  • protected entrapped cells

methodology

1) cell in gelatin microsphere
2) Alginate solution was added. Kept for 20 min
3) CaCl2 added for 20 min to initiate the external Ca2+ cross-linking of the peripheral alginate layer

Alginate-calcium solidification.jpg

Paper for encapsulation in alginate and acidity tests


alginate–chitosan–alginate (ACA)

one of the most widely studied microcapsules for its good biocompatibility and the low cost of chitosan

By having alginate crosslinked with other molecules, this reduces the leakage of drugs from the microcapsule


methodology

1) microbial cells obtained by high speed centrifugation were suspended in 1.5% alginate solution 2) The cell-alginate suspension was extruded into 100 mM CaCl2 solution 3) The microbeads were hardened for 30 min. Chitosan solution was added. 4) alginate solution was added to counteract excess charges on the membranes

Metabolic activity of E.coli in ACA E.coli metabolic activity in ACA.jpg


an innovative method for cultivating microorganisms by encapsulating them within agar spheres
Alginate and chitosan review