Tuesday 21st July 2009
- Produce an enzyme (protein)
- How much? How long for? (GFP as a reporter gene?)
- Be able to control/regulate
- Cell proliferation?
- Do not secrete out of cell
- Switch off once desired compound is produced
- Induce encapsulation
- Adequate mechanical/chemical deposition (bacterial control)
- Acid resistance (has to bypass stomach - capsule composition)
- Release in small intestine (module 5 delivery)
- Non toxic
- Be able to control & regulate; thickness, uniformity
- Time taken for encapsulation to take place (want ‘fast’ encapsulation)
- 100% killing (high as possible killing rate)
- What is the probability of survival? (how do we know it has worked?)
- Failsafe mechanism (use timer?) vs double killing mechanism
- Do not want proteases to degrade our product
- Destruction of cell membrane? (to release our product)
- Non-toxic, fast, reliable
- when do we want to induce killing? Run in parallel? Overlap? Timer?
- how to induce? Light? Timer? Can’t be chemical
- pre-set (decide time to preset)
- module 1 → module 2
- module 2 → module 3
-delay timer for fail safe?
- module 5??
Ranking 1-3; most important(1) to least important (3).
- Sequence available 1
- Expertise/equipment available in College 1
- Stable - know production vs. degradation rates 2
- Compatible with available chassis 1
- Genes/pathway known 1
- Enzyme - specificity? Single enzyme, no crosstalk 1
- Functions in gut temp./environment. Survives and is absorbed 1
- No existing cheap solution → want advantage / benefit 1
- Optimum level/ dose control → does it naturally oscillate with the day? 1
- Impact, appeal, wow factor, high profile 2
- Not covered by previous iGEM teams 3
Currently under construction: