IGEM:IMPERIAL/2009/Genes

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Genes

Presence of restriction enzyme sites

EcoRI XbaI SpeI PstI
PAH
0
1
0
1
Cellulase
2
0
0
0
RcsB
0
0
0
1
B3023
0
0
0
0
WaaL
0
0
0
0
OtsA
0
0
0
0
OtsB
0
0
0
0
Dam
0
0
0
0
TaqI
0
0
0
0

Primers for Site Directed mutagenesis

NB: The website allowing to make primers for these kits has changed an is now: [1]

<b>PAH XbaI

          t201a	                5'-cctgacccacattgaatccagaccttctcgtttaaag-3'
          t201a_antisense              5'-ctttaaacgagaaggtctggattcaatgtgggtcagg-3'

PAH PstI

          g702t	                5'-gaagacgtttctcaattccttcagacttgcactggtttcc-3'
          g702t_antisense              5'-ggaaaccagtgcaagtctgaaggaattgagaaacgtcttc-3'

Cellulase EcoRI site 1

          c951t	                5'-ggctgtaattatcggagaatttggaaccattgacaagaac-3'
          c951t_antisense	        5'-gttcttgtcaatggttccaaattctccgataattacagcc-3'
         

Cellulase EcoRI site 2

          c1323t	                5'-atattttgcgtggcatagaagaatttccaagtcctagcg-3'
          c1323t_antisense	        5'-cgctaggacttggaaattcttctatgccacgcaaaatat-3'

RcsB

          g369c	                5'-ccgaaagctctcgccgcgctccagaaagggaagaaatttacc-3'
          g369c_antisense	        5'-ggtaaatttcttccctttctggagcgcggcgagagctttcgg-3'