IGEM:IMPERIAL/2009/Feedback & Debriefs/Feedback 31 7
From OpenWetWare
Jump to navigationJump to search
Friday 31st of July 2009
Timer & modeling
- the other way of inducing lac repressor is to use
- IPTG is basically on and off, we might have a number of problems
- leakiness in the lac promoter
- the timer is not directly linked
- reduced tunability
- rethink the timer?
- medium defined: put the glucose, put the arabinose, the time delay is directly correlated to the amount of glucose you put in
- how long does it take to make a recombinant protein?
- time delays are massive compared to this
- make the model into a transforming diagram, the new way of communicating the module: in engineering we don't use ODEs, they are hidden in transforms
- what is the Tet repressing well or not?
- it seems so
- Investigating another solution for M1-M2
- use an activator, it would be better
- the current solution wouldn't work (probably)
- Glucose uptake and energy and protein creation
- you can buy it: novagen
- taking two existing prts and making a new one out of it, that is positive, still use an activator
Module 3, Killing
- Good test would be using Dam+ and Dam- strains
- Is the lambda Cl going to interfere with lacI
- Why is harvard's 08 biobrick so bad?
- we should say that at the jamboree
- It has to be a tight promoter
Overview
- Module 1 could take ~5hours
- Module 2 could take ~2hours
- The flow diagrams are good
- we should produce three nice models that we can then link together
- We should probably PCR them all out, much faster
- Using ligation-indipendent cloning can overcome many of the time limitations
- Dam doesn't need synthesis