- 1 Details of thermoinducible system
- 1.1 cI repressor
- 1.1.1 Uploaded cI857 sequence
- 1.1.2 Harvard's characterised clts part
- 1.1.3 clts from pGW7
- 1.1.4 Other plasmids by blasting using Harvard's part
- 1.1.5 Other plasmids from papers
- 1.2 lambda promoter
- 1.3 List of plasmids
- 1.1 cI repressor
Details of thermoinducible system
Uploaded cI857 sequence
Genbank Accession No: E00770
Patent: JP 1986074585-A 8 16-APR-1986
Harvard's characterised clts part
By blasting with cI857 sequence online
Identities = 711/714 (99%)
By blasting with pGW7
100% identity (since gene was taken from pGW7)
4148-4861 (supposed clts gene according to pGW7 plasmid)
clts from pGW7
The gene and plasmid which Harvard took from
the clts gene given as from 4148 to 4861 (same as the part Harvard characterised)
The order of the major features in the plasmid is: EcoRI - partial O - partial cro - lambda R promoter - lambda M promoter - cIts - rexA - rexB - lambda L promoter - HpaI / N - BamHI - SphI - SalI - pMB1 ori – ampR
By blasting with uploaded cI857 sequence
Identities = 870/873 (99%)
4045 to 4917 of pGW7 (27-899 of cI857 gene)
Other plasmids by blasting using Harvard's part
- Escherichia coli plasmid pGBG1
Simple construct containing only TetA gene and cI gene
- Other cloning vectors
pND707/706, pCL476/478, pPL450-452, pBT, pMM200
Other plasmids from papers
Essentially the same as other lambda Pr promoters but stops after OR2.
(-49)TAACACCGTG CGTGTTGACT ATTTTACCTC TGGCGGTGAT AATGGTTGC (-1)
Allows normal growing at up till 30 degrees
TATCACCGCAAGGGATAAATATCTAACACCGTG CGTGTTGACT ATTTTACCTC TGGCGGTGAT AATGGTTGC
Only difference from pAW12 is that it contains a mutation at 41C.
Supposedly allows normal growing at 36 degrees, but promoter strength is weakened.
TATCACCGCAAGGGATAAATATCTAACACCGCG CGTGTTGACT ATTTTACCTC TGGCGGTGAT AATGGTTGC
BLASTED with LacI binding site from Registry (R0010)
No significant similarity found, therefore presumably there is no crosstalk.
Contains both lambda R and lambda L.
Lambda R sequence detected by blasting with pAWJ. The sequence is the same (100% identity).