IGEM:IMPERIAL/2009/Encapsulation/Parts
Module 2 : Gene Sequences and Locations for PCR
G4 : RcsB
G5 : B3023
G6 : WaaL Ligase
G7 + G8 : OtsA and OtsB
Promoter 1
Promoter 1 (P1): BBa_I0500
Specs: Inducible
Notes: Induced by L-arabinose
Present in the 2009 kit
Use modelling to determine how much arabinose should be used
http://partsregistry.org/wiki/index.php?title=Part:BBa_I0500
Promoter 2
Promoter 2 (P2): BBa_I714889
Specs: Constitutive & Repressible
Notes: Works but not present in the 2009 kit
http://partsregistry.org/wiki/index.php?title=Part:BBa_I714889
Promoter 2 (P2): BBa_R0040
Specs: Constitutive & Repressible
Notes: Repressed by TetR. Present in the 2009 kit
http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040
Promoter 2 (P2): BBa_R0053
Specs: Repressible with p22 cII repressor protein(BBa_C0053)
Notes: Not compatible with RBS B0030. This combination yielded very low gfp expression(BBa_I7108)
Present in 2009 kit
http://partsregistry.org/wiki/index.php?title=Part:BBa_R0053
Promoter 2 (P2): BBa_K113001
pT7->cI434 operator ->GFP Specs: Repressible operator
Notes: cI434 operator can repress the gene expression once the concentration of cI434 reaches certain level (threshold detector?) But experience is none and not present in kit
http://partsregistry.org/Part:BBa_K113001
Promoter 3
Promoter 3 (P3): lacI regulated BBa_R0010
Specs: Repressible
Notes: Present in the 2009 kit
http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010
Promoter 3 (P3): lambda cI regulated BBa_R0051
Specs: Repressible
Notes: lambda cI regulated, cI binding results in repression of transcription
Present in the 2009 kit
http://partsregistry.org/Part:BBa_R0051
Promoter 3 (P3): lacI regulated BBa_R0011
Specs: Repressible
Notes: lacI regulated, lambda pL hybrid. repressed by LacI,nd induced by IPTG. (Same as P3)
http://partsregistry.org/Part:BBa_R0011
Promoter 3 (P3): pLacIQ1 promoter BBa_K091112
Specs: Repressible
Notes: Reduces leaky transcription.
Present in the 2009 kit 2
http://partsregistry.org/Part:BBa_K091112
Module 3 : Killing strategy
Module3
Promoter 4 (nonleaky)
T7 promoter system
RBS
RBS 1: BBa_B0034 (Strength = 1) or BBa_B0035 (Strength = 1.124)
Specs: As strong as possible
RBS 2: BBa_B0033 (Strength = 0.01-0.002)
Specs: Weak
RBS 3: BBa_B0035 (Strength = 1.124)
Specs: As strong as possible
RBS 4: BBa_B0034 (Strength = 1)
Specs: Strong
Genes
Gene 1 (G1)
Specs: Protein of interest (e.g. Cellulase or PAH)
Gene 2 (G2) BBa_C0056
Specs: P2 repressor
Notes: Present in the 2009 kit
http://partsregistry.org/wiki/index.php?title=Part:BBa_C0056
Gene 3 (G3) BBa_C0012
Specs: P3 repressor
Notes: Present in the 2009 kit, LacI repressor
http://partsregistry.org/Part:BBa_C0012
Gene 3 (G3) BBa_C0051
Specs: P3 repressor
Notes: Present in the 2009 kit, cI repressor
http://partsregistry.org/wiki/index.php/Part:BBa_C0051
Do you want an RBS?
If you do, follow this link http://partsregistry.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Community_Collection