IGEM:IMPERIAL/2008/Calendar/2008-8-5
<calendar> name=IGEM:IMPERIAL/2008/Calendar date = 2008/07/29 view=threemonths format=%name/%year-%month-%day weekstart=2008/07/01 </calendar>
Briefing
- Yanis Journal Club today (1400) - Environmental Sensing, Chris Wright
- Drylab will be continuing with tutorials
- Wetlab will order sequences and reagents today
- Testing bacterial synthesis... How do we know our material is being secreted rather than just the cells being lysed on production?
- Produce without signal sequence
- Tack on a reporter that is not secreted - if that turns up on the gel with the biomaterial we've got lysis
- Protocols: James, Chris, Tom & Krupa will split into two groups - Cloning and B. subtilis culturing
- Each will organise the relevant protocols, but we'll all pitch in for the actual wetlab work
- Testing bacterial synthesis... How do we know our material is being secreted rather than just the cells being lysed on production?
- QQ will be away for 2 weeks, possibly longer
- Email for microscope training to be sent today
- Microscope work will require a motile E. coli strain
- Journal Clubs coming up:
- Thursday: Erika & Clinton - T7 Promoters
- Friday: Krupa & James - Craig Venter
- Monday: Chris and Prudence - Quorum Sensing
- Tuesday: Tom and Yanis - Division and death in bacteria
Debrief
Vincent put up a list of things he wants to know about... sometime... It's a good way to structure our work, anyhow. It's pasted below, please put your names next to things you'll take care of! Basically I've made a few pages (linked in the Navbar) for each team, and we need to put up concise explanations and summaries of progress. Eventually we'll probably copy and paste a lot of this to the official wiki; so when you're doing internal links, please call them Team:Imperial_College/PageName. That way when we copypasta everything over, pages will already point to the right place... Oh, and if you make a new page, remember to put {{Imperial/StartPage}} at the top and {{Imperial/EndPage}} at the bottom. [:
"Hi iGEMers,
I hope you are all doing well. I would be really interested to catch-up with the iGEM project.
Before we meet again, could someone send me a list of links from your OWW wiki related to the following topics (please no .doc or .pdf as they are not collaboration friendly):
1- Design of your final construct + basic description of its expected behaviour (no sequences needed) - Tom
2- Design of your test constructs + basic description of their use (no sequences needed)- James
3 - Protocols: - wet lab group
+ b.subtilis culture + b.subtilis comptent cell preparation + b.subtilis DNA chromosomal integration + b.subtilis and time lapse microscopy + b.subtilis motility assay + test construct protocols + others that I have missed ?
4 - Your wet lab shopping list - james
5 - Update on the motility analysis - erika
6 - Update on the biomaterial production - prudence
7 - Update on the light sensing system - clinton
8 - description of the different working groups (people involved and scope of the working group) -
9 - Your current planning for the project and sub-projects (deadlines and deliverables)
10 - Also it would be great if you could also comment on the current challenges you are facing in the project - everyone
thanks and see you soon,
Vincent."