IGEM:IMPERIAL/2007/Projects/Hrp System/Testing/Protocol Hrp

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Equipment

  • Twinkle Plate Reader Fluorometer
  • 96 well plate
  • Eppendorf Tubes
  • Gilson Pippettes
  • Plastic Curvetts
  • Incubator 37o shaker
  • Vortex and Centrifuge
  • Spectrometer

Materials and Reagents

  • M9 minimal media 50μg/ml Ampicillin
  • Inducer of promoter at varying concentrations
  • Recombinant acGFP of known concentration
  • Agar Plates
  • E.coli strain containing vector

Protocols

Promoter Test Construct

The promoter pLux is to be tested at 8 varying ranges of inducer concentration.

  • pLux will have a range of 0 to 100μM AHL added at intervals of 12.5μM Protocol

Device Test Construct

For this test construct we initially need to run a preliminary test in order to find out the time intervals and input ranges we need. We will start the preliminary test using the range of concentrations used initially for promoters.