IGEM:IMPERIAL/2007/Projects/Cell by date/Implementation

Cell by Date: Implementation

• Introduction
• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation
• Notes
• References

Chassis Focus

Chassis	Phase 1	Phase 2	Phase 3
E.coli	X
In-Vitro	X	X	X
In-Veso	X	X	X

Test Constructs

Type of Promoter	Test Constructs	In vivo	In Vitro	In veso	Status
Constitutive T7 Promoter	PT7 promoting GFP	Available	Available		Stock Availability: Midiprepped (Tested) Submission to Registry: Available
Constitutive E.coli Promoter	Ptet</sub promoting GFP	Available	Available		Stock Availability: Midiprepped (Tested) Submission to Registry: Available
Inducible E.coli Promoter	PBad promoting GFP	Cancelled	Cancelled	Cancelled	Stock Availability: Cancelled Submission to Registry: N/A
Constitutive E.coli Promoter	PcI promoting GFP	N/A	N/A	N/A	Stock Availability: Not Working Submission to Registry: N/A
Inducible E.coli Promoter	pBad promoting GFP and pcI promoting araC	Cancelled	Cancelled	Cancelled	Stock Availability: Cancelled Submission to Registry: N/A

Ligation Table

More details on building the constructs.

Lab Notebook

• Week 5 (6 Aug): Building Constructs
  
• Week 6 (13 Aug): Building Constructs / Phase 1
  
• Week 7 (20 Aug): Phase 1 / Phase 2
  
• Week 8 (27 Aug):
  
• Week 9 (3 Sep):
  
• Week 10 (10 Sep):
  

<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/15 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>