From OpenWetWare
Jump to: navigation, search

<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/24 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Testing of Homemade S30 Cell Extract

  1. Set up 4 reactions with the following composition
    • S30 Cell extract 16.2μl
    • Reaction buffer 30μl
    • Pyruvate kinase 3.1μl
    • rNTPs 1μl
    • DNA/ddH20 4μl
    • ddH20 5.7μl
  2. Left at 37°C incubator
    • 1. Cell extract reaction with pTet-GFP
    • 2. Cell extract reaction without pTet-GFP (negative control)
    • 3. Cell extract reaction with pT7-GFP
    • 4. Cell extract reaction without pT7-GFP (negative control)